Difference between revisions of "Documentation/Nightly/Extensions/IASEM"

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##Apply the Resampling.
##Apply the Resampling.
|[[Image:TransformModule.png|thumb|Transform Settings]]
|[[Image:TransformModule.png|thumb|Transform Settings: Creating Active Transform "identity"]]
|[[Image:ResampleSettings.png|thumb|Resample settings.]]
|[[Image:ResampleSettings.png|thumb|Resample settings.]]

Revision as of 18:06, 6 August 2013

Home < Documentation < Nightly < Extensions < IASEM

For the stable Slicer documentation, visit the 4.10 page.

Introduction and Acknowledgements

Acknowledgments: This work is supported by NLM, and the Slicer Community.
Author: Bradley Lowekamp
Contact: Bradley Lowekamp <email>blowekamp@mail.nih.gov</email>
License: Apache License 2.0

The National Library of Medicine  
National Alliance for Medical Image Computing (NA-MIC)  

Extension Description

This extension is a compilation of modules which are useful for segmentation and processing of IASEM Electron Microscopy images. It adds modules which help in general with segmenting large data sets, and has components to deal with images in multi-resolutions.

IASEM stands for ion-abrasion scanning electron microscopy, some times also call dual-beam or focused ion-beam scanning electron microscopy (FIBSEM).


  • BinShrink Image Filter
  • SpacingAwareCurvatureDiffusion Image Filter
  • DoubleThreshold Editor Effect
  • BinaryWatershed Editor Effect

Use Cases

BinShrink Image Filter

Bin Shrink is one of the modules used to help ease the process of segmentation. By stipulating a neighborhood size, 5x5x5 for example, it will average the neighboring pixels within that 5x5x5 region and turn them into one pixel. Applying the Bin Shrink will result in reduced resolution and a less noisy image. Based on the features you want to segment, you can apply a more aggressive bin or a less aggressive bin based on the complexity of your features of interest. Binning the image does not significantly affect the quality of the image, it also reduces the amount of memory the computer needs to process the data, so the more an image is binned the faster slicer will work and the more free memory you will have.

Original gray scale image.
After running the BinShrink by a factor of 5x5x5.

SpacingAwareCurvatureDiffusion Image Filter

DoubleThreshold Editor Effect

BinaryWatershed Editor Effect

Walk Through

BinShrink Image Filter

Step 1: Original gray scale image.
Step 2: BinShrink settings.
Step 3: After running the BinShrink by a factor of 5x5x5.
Step 4: ROI Toolbar.
Step 5: Cropped gray scale image.
Step 6: Threshold of the cropped gray scale image.
  1. Load gray scale data.
  2. Go to the Modules search bar, search and select "BinShrink". Go to the BinShrink settings, change the input to the original gray scale image and change the output to "create a new volume".
  3. Apply the BinShrink Image Filter.
  4. Create a Region of Interest (ROI) using the Fiducial Module in the Slicer Toolbar.
  5. Use the Crop Volume module to crop the region of interest.
  6. Apply Threshold Effect, or any other editing technique, to your gray scale image to create a label map.
  7. Resample the label map so its dimensions match those of the gray scale image.
    1. Using the Transforms module, create a new transform and rename it "Identity".
    2. Search and select "Resample Image (BRAINS)" in the modules search bar. Go to the Resample settings to resample the label map.
    3. The Image to Warp should be the label map.
    4. The Reference Image should be the BinShrink gray scale image.
    5. Create a new Output Image.
    6. Change the pixel type to "short".
    7. Change the Transform file to "Identity".
    8. Change the Interpolation Mode to "NearestNeighbor".
    9. Apply the Resampling.
Transform Settings: Creating Active Transform "identity"
Resample settings.


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