<?xml version="1.0"?>
<feed xmlns="http://www.w3.org/2005/Atom" xml:lang="en">
	<id>https://www.slicer.org/w/index.php?action=history&amp;feed=atom&amp;title=Slicer3%3AFluorescence_and_Electron_Microscopy_Support</id>
	<title>Slicer3:Fluorescence and Electron Microscopy Support - Revision history</title>
	<link rel="self" type="application/atom+xml" href="https://www.slicer.org/w/index.php?action=history&amp;feed=atom&amp;title=Slicer3%3AFluorescence_and_Electron_Microscopy_Support"/>
	<link rel="alternate" type="text/html" href="https://www.slicer.org/w/index.php?title=Slicer3:Fluorescence_and_Electron_Microscopy_Support&amp;action=history"/>
	<updated>2026-04-11T21:12:10Z</updated>
	<subtitle>Revision history for this page on the wiki</subtitle>
	<generator>MediaWiki 1.33.0</generator>
	<entry>
		<id>https://www.slicer.org/w/index.php?title=Slicer3:Fluorescence_and_Electron_Microscopy_Support&amp;diff=4419&amp;oldid=prev</id>
		<title>Marianna at 20:10, 19 May 2008</title>
		<link rel="alternate" type="text/html" href="https://www.slicer.org/w/index.php?title=Slicer3:Fluorescence_and_Electron_Microscopy_Support&amp;diff=4419&amp;oldid=prev"/>
		<updated>2008-05-19T20:10:19Z</updated>

		<summary type="html">&lt;p&gt;&lt;/p&gt;
&lt;table class=&quot;diff diff-contentalign-left&quot; data-mw=&quot;interface&quot;&gt;
				&lt;col class=&quot;diff-marker&quot; /&gt;
				&lt;col class=&quot;diff-content&quot; /&gt;
				&lt;col class=&quot;diff-marker&quot; /&gt;
				&lt;col class=&quot;diff-content&quot; /&gt;
				&lt;tr class=&quot;diff-title&quot; lang=&quot;en&quot;&gt;
				&lt;td colspan=&quot;2&quot; style=&quot;background-color: #fff; color: #222; text-align: center;&quot;&gt;← Older revision&lt;/td&gt;
				&lt;td colspan=&quot;2&quot; style=&quot;background-color: #fff; color: #222; text-align: center;&quot;&gt;Revision as of 20:10, 19 May 2008&lt;/td&gt;
				&lt;/tr&gt;&lt;tr&gt;&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot; id=&quot;mw-diff-left-l1&quot; &gt;Line 1:&lt;/td&gt;
&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot;&gt;Line 1:&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;−&lt;/td&gt;&lt;td style=&quot;color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;  Back to [&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;[&lt;/del&gt;NA-MIC_Collaborations&lt;del class=&quot;diffchange diffchange-inline&quot;&gt;|&lt;/del&gt;NA-MIC_Collaborations]]&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;  Back to [&lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;http://www.na-mic.org/Wiki/index.php/&lt;/ins&gt;NA-MIC_Collaborations NA-MIC_Collaborations]]&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;= Name of Template project goes here =&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;= Name of Template project goes here =&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot; id=&quot;mw-diff-left-l34&quot; &gt;Line 34:&lt;/td&gt;
&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot;&gt;Line 34:&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt; &lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class='diff-marker'&gt;−&lt;/td&gt;&lt;td style=&quot;color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;[[Image:SlicerAstrocyte.jpg | NCMIR image processing pipeline]]&lt;/div&gt;&lt;/td&gt;&lt;td class='diff-marker'&gt;+&lt;/td&gt;&lt;td style=&quot;color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;[[Image:SlicerAstrocyte.jpg&lt;ins class=&quot;diffchange diffchange-inline&quot;&gt;|left|thumb|450px&lt;/ins&gt;| NCMIR image processing pipeline]]&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;/table&gt;</summary>
		<author><name>Marianna</name></author>
		
	</entry>
	<entry>
		<id>https://www.slicer.org/w/index.php?title=Slicer3:Fluorescence_and_Electron_Microscopy_Support&amp;diff=3857&amp;oldid=prev</id>
		<title>Marianna: 1 revision</title>
		<link rel="alternate" type="text/html" href="https://www.slicer.org/w/index.php?title=Slicer3:Fluorescence_and_Electron_Microscopy_Support&amp;diff=3857&amp;oldid=prev"/>
		<updated>2008-05-15T18:01:47Z</updated>

		<summary type="html">&lt;p&gt;1 revision&lt;/p&gt;
&lt;table class=&quot;diff diff-contentalign-left&quot; data-mw=&quot;interface&quot;&gt;
				&lt;tr class=&quot;diff-title&quot; lang=&quot;en&quot;&gt;
				&lt;td colspan=&quot;1&quot; style=&quot;background-color: #fff; color: #222; text-align: center;&quot;&gt;← Older revision&lt;/td&gt;
				&lt;td colspan=&quot;1&quot; style=&quot;background-color: #fff; color: #222; text-align: center;&quot;&gt;Revision as of 18:01, 15 May 2008&lt;/td&gt;
				&lt;/tr&gt;&lt;tr&gt;&lt;td colspan=&quot;2&quot; class=&quot;diff-notice&quot; lang=&quot;en&quot;&gt;&lt;div class=&quot;mw-diff-empty&quot;&gt;(No difference)&lt;/div&gt;
&lt;/td&gt;&lt;/tr&gt;&lt;/table&gt;</summary>
		<author><name>Marianna</name></author>
		
	</entry>
	<entry>
		<id>https://www.slicer.org/w/index.php?title=Slicer3:Fluorescence_and_Electron_Microscopy_Support&amp;diff=3856&amp;oldid=prev</id>
		<title>Melonakos at 19:05, 28 November 2007</title>
		<link rel="alternate" type="text/html" href="https://www.slicer.org/w/index.php?title=Slicer3:Fluorescence_and_Electron_Microscopy_Support&amp;diff=3856&amp;oldid=prev"/>
		<updated>2007-11-28T19:05:04Z</updated>

		<summary type="html">&lt;p&gt;&lt;/p&gt;
&lt;p&gt;&lt;b&gt;New page&lt;/b&gt;&lt;/p&gt;&lt;div&gt; Back to [[NA-MIC_Collaborations|NA-MIC_Collaborations]]&lt;br /&gt;
&lt;br /&gt;
= Name of Template project goes here =&lt;br /&gt;
&lt;br /&gt;
Analyze dendritic spine morphologies from electron tomographic reconstructions.&lt;br /&gt;
&lt;br /&gt;
= Description =&lt;br /&gt;
&lt;br /&gt;
Dendritic spines are the primary sites of excitatory neuronal communication in the brain.  It is of fundamental interest to cellular neurobiologists to understand how the number, size, and shape of dendritic spines varies across brain regions, and as a function of experimental treatment within a given region.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
''Objective (Light Microscopy)'' Incorporate into Slicer our existing microscopy processing and analysis routines, currently being done in Matlab.&lt;br /&gt;
&lt;br /&gt;
''Progress''&lt;br /&gt;
Reading 3-D TIFF images and generating surface models has been easily accomplished.&lt;br /&gt;
&lt;br /&gt;
* 2007-04-26: Modifying Otsu Segmentation CLM to make use of Connected Components Image Filter.&lt;br /&gt;
** Works in Linux, not OS X.&lt;br /&gt;
* 2007-04-25: Discussion of relevant steps to accomplish initial astrocyte parsing project.&lt;br /&gt;
** Using EM Segmentation or 2-D histogram normalization approach to segment immunostaining data.&lt;br /&gt;
** Need 3-D erosion functionality for 'shrinking' astrocyte image volume to separate into two domains: an outer, &amp;quot;hull&amp;quot;, and an inner, &amp;quot;core&amp;quot; domain in which differential protein distributions will be assessed.&lt;br /&gt;
** Can ParaView be used as visualization tool for surface rendering?  Is it faster?&lt;br /&gt;
&lt;br /&gt;
= Key Investigators =&lt;br /&gt;
&lt;br /&gt;
* NCMIR/UCSD: W. Bryan Smith, Mark Ellisman&lt;br /&gt;
* Isomics: Steve Pieper&lt;br /&gt;
&lt;br /&gt;
= Links =&lt;br /&gt;
&lt;br /&gt;
* A [[Media:NCMIR example dataset.zip | sample dataset]] is available.&lt;br /&gt;
*[http://ncmir.ucsd.edu/~bryan/spines01.jpg A segmented spiny dendrite from cerebellum]&lt;br /&gt;
*[http://ncmir.ucsd.edu/~bryan/spineSample.jpg An example set of segmented spines]&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
[[Image:SlicerAstrocyte.jpg | NCMIR image processing pipeline]]&lt;/div&gt;</summary>
		<author><name>Melonakos</name></author>
		
	</entry>
</feed>